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Fig. 4 | Molecular Medicine

Fig. 4

From: Lack of Interferon-γ Production Despite the Presence of Interleukin-18 During Cutaneous Wound Healing

Fig. 4

Expression pattern of chymase during repair. Total cellular RNA (30 µg) from normal and wounded back skin from Balb/c mice was analyzed by RNase protection assay for expression of mast cell-derived chymase. Sixteen wounds (n = 16) from the backs of four animals were excised for each experimental time point and used for RNA isolation. The time after injury is indicated at the top of each lane. Control skin refers to nonwounded skin of mice. One thousand cpm of the hybridization probe were added to the lane labeled probe. One representative experiment is presented. Expression of GAPDH mRNA is shown as a loading control.

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Molecular Medicine

ISSN: 1528-3658