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Fig. 5 | Molecular Medicine

Fig. 5

From: Lack of Interferon-γ Production Despite the Presence of Interleukin-18 During Cutaneous Wound Healing

Fig. 5

TGF-β1 as a negative regulator of IFN-γ release. (A) Freshly isolated PBMC from different donors (donor 1 to 4) were stimulated by PHA (1 µg/ml)/IL-18 (8 ng/ml) for 24 hr in the presence or absence of TGF-β1 (10 ng/ml) to investigate IFN-γ release from the cells. Interferon-γ secreted into the cell culture supernatants was determined by ELISA. Control represents the conditions for unstimulated PBMC. The varying amounts of secreted IFN-γ were due to interindividual differences. (B) Total cellular RNA (20 µg) from normal and wounded back skin was analyzed by RNase protection assay for expression of TGF-β1. The pattern of TGF-β1 mRNA expression in normal Balb/c mice (upper panel, indicated as wt), or diabetic animals (lower panel, indicated as db) is shown. Sixteen wounds (n = 16) from the backs of four animals were excised for each experimental time point and used for RNA isolation. The time after injury is indicated at the top of each lane. Control skin refers to nonwounded skin of mice. One thousand cpm of the hybridization probe were added to the lane labeled probe. Expression of GAPDH is shown as a loading control.

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