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Table 1 Quantification of IFN-γ protein expression in wound lysates isolated from normal and diabetic animals

From: Lack of Interferon-γ Production Despite the Presence of Interleukin-18 During Cutaneous Wound Healing

IFNγ-Content

ctrl skin (wt, db)

< d.l.

1, 3, 5, 7, 13 d

< d.l.

wound (wt, db)

 

1d wound (25 µg)

 

+50 pg rec. mlFNγ

61 pg

  1. Total protein (25 µg) from lysates of nonwounded and wounded back skin (day 1, 3, 5, 7, and 13 after injury as indicated) isolated from Balb/c mice (wt) and diabetic mice (db) were assessed for the total amount of IFN-γ-specific proteins dring wound repair as determined by ELISA (R&D Systems). Eight wounds (n = 8) from the backs of four animals were excised for each experimental time point and used for protein isolation. Addition of 50 pg recombinant murine IFN-γ to 1-day wound lysates was used to verify the functionality of the IFN-γ-ELISA. < d.1., below detection limit.