IFNγ-Content |
---|
ctrl skin (wt, db) | < d.l. |
1, 3, 5, 7, 13 d | < d.l. |
wound (wt, db) | |
1d wound (25 µg) | |
+50 pg rec. mlFNγ | 61 pg |
- Total protein (25 µg) from lysates of nonwounded and wounded back skin (day 1, 3, 5, 7, and 13 after injury as indicated) isolated from Balb/c mice (wt) and diabetic mice (db) were assessed for the total amount of IFN-γ-specific proteins dring wound repair as determined by ELISA (R&D Systems). Eight wounds (n = 8) from the backs of four animals were excised for each experimental time point and used for protein isolation. Addition of 50 pg recombinant murine IFN-γ to 1-day wound lysates was used to verify the functionality of the IFN-γ-ELISA. < d.1., below detection limit.