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Fig. 2 | Molecular Medicine

Fig. 2

From: Tumor Necrosis Factor and Reactive Oxygen Species Cooperative Cytotoxicity Is Mediated via Inhibition of NF-κB

Fig. 2

Synergistic effect of tumor necrosis factor alpha (TNFα) and H2O2 on the number of TUNEL-positive cells. (A) Human brain capillary endothelial cells, and (B) rat brain capillary endothelial cells were treated with either TNFα (photomicrographs a and d) or H2O2 (photomicrographs b and e) or with both agonists (photomicrographs c and f) for 24 hr and apoptotic nuclei were visualized with TUNEL assay as described in Materials and Methods. Photomicrographs are representatives of 15 images captured for each condition. The top row of panels (A) and (B) (photomicrographs a, b, and c) are phase-contrast images, the bottom row of panels (A) and (B) (photomicrographs d, e, and f) are corresponding binary images. Apoptotic nuclei are indicated with arrows.

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