Fig. 1

Correlation between superoxide anion (A) or nitric oxide (B) production and FcγR expression after IC-stimulation. As already described in the Materials and Methods section, J774.A1 cells (2 × 10⁶ cells/ml) were stimulated for 2 days in the presence of IFN-γ (1,000 U/ml) and then cells were incubated in the presence of IgG₁/anti-IgG₁ (100/20 µg/ml), the generation of superoxide anion being assayed by reduction of ferricytochrome c (A), whereas nitric oxide production (B) was evaluated after measurement of \({\rm{NO}}_2^ - \) production using the Griess reaction, the expression of Fcγ receptor (CD16/CD32) expression was done using the 2.4G2 mAb (rat IgG2b) and cell-related fluorescence intensity (MFI) was detected by flow cytometry using a FACScan system (Becton Dickinson, Grenoble, France). Data are from six independent experiments, and correlation with FcγR expression was evaluated using the Spearman rank test.