Fig. 6

Effect of a SOD mimetic and of an inhibitor of protein synthesis on the (A) Bo-SOD1-induced iNOS expression, and (B) tumor necrosis factor-α (TFN-α) production. As described in Materials and Methods, IFN-γ-activated J774.A1 cells were stimulated or not for 24 hr with IgG1 IC or specific Bo-SOD1 IC (20 µg of anti-Bo-SOD1 + 100 µg of Bo-SOD1) and in the presence or absence of MnTBAP (50 µM) or cycloheximide (50 µM). Then (A) iNOS expression or, (B) TNF-α production were evaluated and data represent the mean ± SEM of three different experiments.