Interferon-γ-Treated Cells+ | Mn-SOD | Glutathion Peroxidase (U/mg/protein) | Catalase | Glutathion nmoles/106 Cells |
---|
None | 75 ± 2 | 9.3 ± 1 | 870 ± 31 | 22 ± 3 |
L-NAME | 7 ± 1 | 9.1 ± 2 | 520 ± 15 | 15 ± 2 |
SOD1 | 12 ± 2 | 8.4 ± 1 | 695 ± 25 | 19 ± 1 |
SOD1 IC | 212 ± 1 | 15.5 ± 2 | 920 ± 15 | 30 ± 3 |
SOD1 IC + L-NAME | 85 ± 2 | 29.0 ± 2 | 1025 ± 30 | 55 ± 4 |
- Interferon-γ-treated cells (2 X 106 cells/ml) were challenged overnight by Bo-SOD1 IC (20 µg of anti-Bo-SOD1 and 10 µg of Bo-SOD1) and/or of 1 mM L-NAME. After extraction of total intracellular proteins, antioxidant enzymes activities and glutathion measurements were done as described in Materials and Methods. Data are the mean ± SD (triplicate samples) of one representative experiment out of four.