Fig. 5

MIF expression in the 38C13 B cell lymphoma. C3H/HeN mice were administered an i.d. injection of 38C13 tumor cells. After 7 days the tumors were excised and then fixed, and adjacent sections were stained with anti-MIF mAb, anti-vWF mAb, or an isotype control (A). The primary antibody binding was detected with a peroxidase-linked secondary antibody and 3-amino-9-ethylcarbazole (for anti-MIF) or diaminobenzidine (for anti-vWF) as chromogenic substrate. Double immunostaining with anti-MIF mAb (brown, diaminobenzidine) and anti-vWF mAb (pink, new fuchsin) shows a colocalization of MIF and vWF in endothelium (B).