MIF antisense oligonucleotides inhibit MIF expression and endothelial cell proliferation in vitro. (A). Endothelial cells were transfected with antisense human MIF (AS-MIF) or sense human MIF (S-MIF) oligonucleotides for 20 hr and examined for MIF protein expression by Western blot analysis. RT-PCR for MIF also showed a decrease in MIF mRNA expression by AS-MIF (data not shown). (B). Transfected endothelial cells were pulsed with [3H]thymidine for the last 16 hr of transfection and proliferation was analyzed by liquid scintillation counting. Data are expressed as mean ± SD (n = 4) and are representative of one experiment that was performed three times (*p < 0.05).