Fig. 1
From: Peptidomimetic Fluoromethylketone Rescues Mice from Lethal Endotoxic Shock
Structures and caspase-inhibitory activities of compounds IDN 1529 and IDN 1965.
(A) Structures. (B) Inhibitory constants determined against pure, recombinant mouse caspase 1 and human caspases 3, 6, and 8. (C) Inhibition of caspase 1 within primary macrophages. Peptone-elicited mouse peritoneal macrophages (106/well) were stimulated with LPS (10 ng/ml) for 4 hr in the presence of indicated concentrations of inhibitor. Cells were then exposed to nigericin (10 µM) for 20 min. IL-1β in the conditioned media was measured by ELISA. With vehicle instead of inhibitor, IL-1β averaged 14.8 ng/ml. Results are from a representative experiment performed in duplicate.