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Fig. 1 | Molecular Medicine

Fig. 1

From: Protection Against Hyperacute Xenograft Rejection of Transgenic Rat Hearts Expressing Human Decay Accelerating Factor (DAF) Transplanted into Primates

Fig. 1

Analysis of transgene integration in transgenic rats. (A) Design of ICAM2DAF cDNA construct used for microinjection (not drawn to scale). (B) Southern blot from transgenic rats (DAF+/−). Genomic DNA (10 µg) was digested by BamHI, separated through 1 % agarose gel electrophoresis, and blotted. Known copy numbers of the microinjected fragment diluted into genomic DNA from a nontransgenic rat were used as controls. Blot was hybridized with a α 32P-dCTP labeled cDNA probe encoding the microinjected cDNA sequences (3.1 kb). BamHI releases a 3.1 kb fragment (arrow).

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