Fig. 1From: Protection Against Hyperacute Xenograft Rejection of Transgenic Rat Hearts Expressing Human Decay Accelerating Factor (DAF) Transplanted into PrimatesAnalysis of transgene integration in transgenic rats. (A) Design of ICAM2DAF cDNA construct used for microinjection (not drawn to scale). (B) Southern blot from transgenic rats (DAF+/−). Genomic DNA (10 µg) was digested by BamHI, separated through 1 % agarose gel electrophoresis, and blotted. Known copy numbers of the microinjected fragment diluted into genomic DNA from a nontransgenic rat were used as controls. Blot was hybridized with a α 32P-dCTP labeled cDNA probe encoding the microinjected cDNA sequences (3.1 kb). BamHI releases a 3.1 kb fragment (arrow).Back to article page