Fig. 4

FACS analysis of human DAF expression by ECs from hemizygous and homozygous transgenic rats. Endothelial cells were isolated from heart of wild-type (WT) and transgenic rats, either hemizygous (DAF^+/−) or homozygous (DAF^+/+). Immunostaining was performed using an anti-human DAF MAb to compare levels of DAF expression on ECs. An anti-rat ICAM-1 MAb and anti-human von Willebrand factor (vWf) antibody were used to assess the purity of EC preparations. Fluorescence was measured on 10,000 cells/sample using a FACS-calibur. Data are depicted in histograms plotting mean fluorescence intensity (MFI) on a log scale (x-axis) versus cell number (y-axis).