Protection Against Hyperacute Xenograft Rejection of Transgenic Rat Hearts Expressing Human Decay Accelerating Factor (DAF) Transplanted into Primates

Table 2 Immunohistochemical analysis of cardiac xenografts for human Ig and complement deposition

Rats	Human DAF	vWf	Human IgG	Human IgM	C3	C4	C9	Factor B
Ungrafted wild type (n = 3)	−	+ +	−	−	−	−	−	−
Wild type (n = 3)	−	+ + +	+ +	+	+ +	+/+ +	+ +	−
DAF^+/−transgenic (n = 3)	+ + +	+ + +	+ +	+	+	+	−/+	−
DAF^+/+ transgenic (n = 2)	+ + + +	+ + +	+ +	+	+	+	+/−	−

Immunostaining of xenograft cryosections was performed as described in Materials and Methods. Results are expressed as a mean of experiments performed on two (DAF^+/+ transgenics) or three (ungrafted, wild-type, and DAF^+/−transgenic) animals. −, no staining; +, low intensity; ++, medium intensity; + + +, high intensity; + + + +, very high intensity.

ISSN: 1528-3658