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Table 4 Quantitative RT-PCR of genes that are involved in islet biology

From: Anx7 Is Required for Nutritional Control of Gene Expression in Mouse Pancreatic Islets of Langerhans

Gene Islets Liver
GKRS1 (glucokinase, related sequence 1) −1.06 ± 0.17 −2.53 ± 0.31*
PTEN (phosphatase and tensin homolog) −21.25 ± 0.17* 3.94 ± 0.092*
GLUT2 (glucose transporter isoform 2) −5.06 ± 0.21* 3.16 ± 0.035*
PDX 1 (pancreatic and duodenal homeobox) −4.56 ± 0.14* 0.00
IGF 1 (insulin-like growth factor 1) −2.57 ± 0.13* 5.42 ± 0.046*
NEURO D1 (neurogenic differentiation factor 1) −5.58 ± 0.15* 1.91 ± 0.18
NGN 3 (neurogenin 3) 1.18 ± 0.15 −3.53 ± 0.23*
  1. For qPCR results, data are means with SE for relative expression levels (arbitrary units). PCR conditions and the primer pairs used are as described in “Materials and Methods” section. Values are shown as a fold difference between mutant mice (+/−) and their littermate control (+/+), using the comparative Ct method (as described in method).
  2. *p ≤ 0.05, n = 4, (−) = down regulated gene.