Immunoblot analysis of Bax protein expression in liver mitochondrial fraction at 3 and 5 hr post-surgery, with and without vitamin treatment. Lanes 1 and 4: PH-Control. Lane 2: PH-E1 (100 mg/kg body weight). Lane 3: PH-E2 (600 mg/kg body weight). Lane 5: PH-C1 (30 mg/kg body weight). Lane 6: PH-C2 (100 mg/kg body weight). The accompanying bars represent the densitometry expressed in percent from five separate animals sets. PH vitamins-treated animals (gray bars) show a decrease of 30% in Bax protein respect to PH-Control (black bars) considering PH-Control as 100%. *Statistically significant against PH-Control.