Fig. 3

Detection of lethally damaged cells at the boundary of stroke by in situ ligation at 6 and 72 hr after the onset of ischemia. The first dying cells appear at the boundary at the earliest time point of 6 hr. Large numbers of dying cells are demonstrated within the stroke area at a later time of 72 hr post-ischemia. The arrow in the inset indicates the direction of progression of DNA damage and cell death as time goes by. (Bar, 150 µm).