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Fig. 1 | Molecular Medicine

Fig. 1

From: Angiotensin II-Induced Mesangial Cell Apoptosis: Role of Oxidative Stress

Fig. 1

(A and B) Effect of ANG II on MC apoptosis. Equal numbers of MCs were incubated in media containing either buffer or ANG II (10−6 M) for 24 hr. Subsequently, cells were stained with H-33342 and propidium iodide. (A) Control cells. An apoptotic cell shows bright fluorescence (arrow). (B) ANG II-treated cells. Apoptotic cells show bright fluorescence (arrows) and necrosed cells are stained pink (arrowhead). (C and D) Equal number of MCs were incubated in media containing either buffer (control) or ANG II (10−8 M) for 24 hr. Subsequently, cells were assayed for apoptosis by TUNEL method. (C) Control cells. (D) ANG II-treated cells. Nuclei of apoptotic cells are darkly stained (arrows). (E and F) Effect of ANG II on MC nitrotyrosine labeling. Equal number of cells, grown in chamber slides, incubated in media containing either buffer (control) or ANG II (10−8 M for 24 hr). Subsequently, cells were immunostained for nitrotyrosine. (E) Control cells. (F) ANG II-treated cells. Brown staining (arrows) indicates labeling for nitrotyrosine.

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