Fig. 2
From: Angiotensin II-Induced Mesangial Cell Apoptosis: Role of Oxidative Stress
(A) Dose-response effect of Ang II on mesangial cell apoptosis. Equal numbers of MCs were incubated in media containing either vehicle (control) or variable concentrations of ANG II (10−10, 10−8, and 10−6 M) for 24 hr. At the end of the incubation, cells were stained with H-33342 and PI. Results (means ± SEM) are from four sets of experiments, each carried out in triplicate. Intergroup comparisons were performed by analysis of variance. A Newman-Keuls multiple range test was used to calculate a q value. *p < 0.001 compared with respective control and ANG II (10−10 M); **p < 0.001 compared with respective ANG II (10−8 M); ***p < 0.001 compared with respective control and ANG II (10−10 M). (B) Effect of ANG II on mesangial cell DNA fragmentation. Equal numbers of MCs were incubated in media containing either vehicle (control) or variable concentrations of ANG II (10−10, 10−8, and 10−6 M) for 24 hr. Subsequently, cells were washed, DNA extracted, and electrophoresed. Lanes 1 and 6 are molecular markers. Lanes 2 and 3 are control and ANG II (10−10 M), respectively. ANG II 10−8 and 10−6 M are shown in lanes 4 and 5, respectively. ANG II in concentrations 10−8 and 10−6 M induced DNA fragmentation in the form of a ladder pattern. (C) Effect of anti-TGF-β antibody on ANG II-induced mesangial cell apoptosis. Equal numbers of MMCs were incubated in media containing either vehicle (control), ANG II (10–8 M), or anti-TGF-β antibody (1 µg/ml) + ANG II for 24 hr. Cells were then assayed for apoptosis. Results (means ± SEM) are from four series of experiments, each carried out in triplicate. *p < 0.001 compared with control and ANG II + anti-TGF-β antibody; **p < 0.01 compared with control. (D) Evaluation of the effect of L-NAME on ANG II-induced mesangial cell apoptosis. Equal numbers of MMCs were incubated in media containing buffer (control), ANG II (10−8M), L-NAME (1 mM), or L-NAME + ANG II for 24 hr. Cells were then stained for apoptosis. Results (means ± SEM) are from four series of experiments, each carried out in triplicate. *p < 0.001 compared with control and ANG II + L-NAME.