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Fig. 3 | Molecular Medicine

Fig. 3

From: Angiotensin II-Induced Mesangial Cell Apoptosis: Role of Oxidative Stress

Fig. 3

Role of oxidative stress in ANG II-induced mesangial cell apoptosis. (A) Effect of antioxidants on ANG II-induced mesangial cell apoptosis. Equal numbers of MMCs were incubated in media containing buffer (control), DPI (10 µM), ascorbic acid (AA, 100 µM), or NAC (50 µM) with or without ANG II (10−8 M) for 24 hr. Subsequently, cells were stained with H-33342 and PI. Results (means ± SEM) are from four series of experiments, each carried out in triplicate. *p < 0.001 compared with control, ANG II + DPI, ANG II + AA, or ANG II + NAC; **p < 0.001 compared with control and respective DPI and NAC; ***p < 0.001 compared with ANG II + DPI and ANG II + NAC. (B) Effect of propranolol on ANG II-induced mesangial cell apoptosis. Equal numbers of MCs were preincubated in media containing either buffer or propranolol (100 µM) for 16 hr followed by incubation with either buffer or ANG II (10−8 M) for 16 hr. Subsequently cells were assayed for apoptosis. *p < 0.001 compared with all other variables. (C) Effect of hemin and zinc protoporphyrin on ANG II-induced MC apoptosis. Equal numbers of MCs were preincubated in media containing either buffer (control), hemin (5 µM), or zinc protoporphyrin (Zn-P, 50 µM) for 16 hr followed by incubation in media containing either buffer or ANG II (10−8 M) for 16 hr. Subsequently, cells were assayed for apoptosis. Results (means ± SEM) are from eight series of experiments. *p < 0.001 compared with control and hemin + ANG II; **p < 0.05 compared with ANG II alone.

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