Characterization of ED77. (I) Flow cytometry analysis of HLA-G expression in ED77. A shift in fluorescence intensity is observed with ED77 (heavy trace) indicating expression of HLA-G as compared to nonimmune complex in control (light trace). Immunofluorescence for (II) Anti-CD133 1:50 ED77 revealing a punctate expression pattern. (III) Control. (IV) Anti-cytokeratin-7 1:50 in ED77 showing strong widespread staining. (V) Control. (Original magnifications ×400).