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Fig. 3 | Molecular Medicine

Fig. 3

From: Farnesyl Protein Transferase Inhibition Interferes with Activation of MAP Kinase Family Members in Human Peripheral Blood Monocytes

Fig. 3

Effects of farnesyl protein transferase inhibition on p38 MAP kinase activation. FTIs inhibit phosphorylation of p38 MAP kinase after TPA, CSF-1, and LPS stimulation in human peripheral blood monocytes. Cells were either incubated or not incubated with FTI (20 µM) for 16 hr and stimulated afterward with TPA, CSF-1 or LPS for 10 min. Phosphorylation of p38 MAP kinase was measured by Western blot in eight volunteers. Antibody binding was quantified using image analysis software. All laboratory based values are given as means ± SEM. (A) Effects of FTI on TPA-induced p38 MAP kinase phosphorylation. (B) Effects of FTI on CSF-1-induced p38 MAP kinase phosphorylation. (C) Effects of FTI on LPS-induced p38 MAP kinase phosphorylation. (D) A representative example of a Western blot analysis of phosphorylated p38 MAP kinase after TPA stimulation (increasing concentrations in ng/ml) in absence and presence of FTI. (E) A representative example of a Western blot analysis of phosphorylated p38 MAP kinase after CSF-1 stimulation (increasing concentrations in ng/ml) in absence and presence of FTI. (F) A representative example of a Western blot analysis of phosphorylated p38 MAP kinase after LPS stimulation (increasing concentrations in ng/ml) in absence and presence of FTI.

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