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Fig. 1 | Molecular Medicine

Fig. 1

From: Zinc Finger Transcription Factors as Molecular Targets for Nitric Oxide-mediated Immunosuppression: Inhibition of IL-2 Gene Expression in Murine Lymphocytes

Fig. 1

Increasing concentrations of SNOC or H2O2 induce apoptosis in lymphocytes. EL4-6.1 cells were incubated for 3 hr with increasing concentrations of SNOC (A) or of H2O2 (B). Cells were then washed and incubated in fresh medium. After additional 3- or 21-hr culture, apoptosis was quantitated by counting cells with condensed chromatin or with fragmented nuclei after staining with Hoechst 33342. Concentrations of up to 1 mM of SNOC or 0.5 mM of H2O2 did not induce apoptosis. Experiments were performed in triplicate, with at least 102 cells/well. Values are mean ± SD of three independent experiments.

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