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Fig. 1 | Molecular Medicine

Fig. 1

From: Tyrosine Phosphorylation of Focal Adhesion Kinase and Paxillin Regulates the Signaling Mechanism of the Rapid Nongenomic Action of Dexamethasone on Actin Cytoskeleton

Fig. 1

Determination of G-/total actin ratios in serum-starved Ishikawa cells. (A) Cells were preincubated for 90 min with genistein (GEN, 100 µg/ml), followed by a 15-min treatment with 107 M dexamethasone (GEN + DEX). (B) Cells were preincubated for 3 hr with erbstatin analogue (EA, 1 µM), followed by a 15-min treatment with 10−7 M DEX (EA + DEX). (C) Cells were preincubated for 30 min with sodium orthovanadate (1 mM) in conjunction with hydrogen eroxide (3 mM) (pervanadate, PERV), followed by a 15-min treatment with 107 M DEX (PERV + DEX). In A–C, DEX represents the effect of 15-min cell treatment with DEX, 107 M. All results represent mean ± SE. *, significant differences (p < 0.05) of the G-/total actin ratios, compared to untreated cells (control).

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