Fig. 2

Tyrosine phosphorylation (A) and protein levels (B) of FAK in Ishikawa cells treated with DEX. (A) Ishikawa cells were serum starved overnight and then exposed to 10⁻⁷ M DEX for 2, 15, and 30 min. Cell lysates were immunoprecipitated with anti-phosphotyrosine monoclonal antibody and after separation of immunoprecipitates on SD S-Polyacrylamide gradient gel (4–20%), proteins were transferred to a nitrocellulose membrane and immunoblotted with anti-FAK monoclonal antibody. Heavy and light chains of the anti-phosphotyrosine IgG used to immunoprecipitate proteins phosphorylated on tyrosine residues are also shown. IP, immunoprecipitation, WB; Western blot. (B) FAK was immunoprecipitated from Ishikawa cells that were serum starved overnight and then incubated with DEX for 2, 15, and 30 min. After separation of immunoprecipitates on SDS-polyacrylamide gradient gel (4–20%), proteins were transferred to a nitrocellulose membrane and immunoblotted with anti-FAK monoclonal antibody.