Fig. 3From: Tyrosine Phosphorylation of Focal Adhesion Kinase and Paxillin Regulates the Signaling Mechanism of the Rapid Nongenomic Action of Dexamethasone on Actin CytoskeletonTyrosine phosphorylation (A) and protein levels (B) of paxillin in Ishikawa cells treated with DEX. (A) Ishikawa cells were serum starved overnight and then exposed to 10−7 M DEX for 2, 15, and 30 min. Cell lysates were immunoprecipitated with anti-phosphotyrosine monoclonal antibody, and after separation of immunoprecipitates on SDS-polyacrylamide gradient gel (4–20%), proteins were transferred to a nitrocellulose membrane and immunoblotted with anti-paxillin monoclonal antibody. Heavy and light chains of the anti-phosphotyrosine IgG used to immunoprecipitate proteins phosphorylated on tyrosine residues are also shown. IP, immunoprecipitation, WB, Western blot. (B) Ishikawa cells were serum starved overnight and then treated with DEX for 2, 15, and 30 min. After separation on SDS-polyacrylamide gel (12%), proteins were transferred to a nitrocellulose membrane and immunoblotted with anti-paxillin monoclonal antibody.Back to article page