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Fig. 3 | Molecular Medicine

Fig. 3

From: Human Breast Carcinoma Cells Express Type II IL-4 Receptors and Are Sensitive to Antitumor Activity of a Chimeric IL-4-Pseudomonas Exotoxin Fusion Protein in vitro and in vivo

Fig. 3

Displacement analysis of 125I-IL-4 binding to breast cancer cells. This was performed on MDA-MB231 and MCF-7 cell lines (upper panel) and R-BT breast cancer primary cell culture (lower panel). Specific binding data was utilized to generate the Scatchard curves. Typically, for these experiments, a single cell suspension of tumor cells was incubated for 2 hr with increasing concentrations of unlabeled excess IL-4 in the presence of a fixed concentration (100–200 pM) of 125I-IL-4 at 4°C. Bound radioactivity was determined as described in the materials and methods section.

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Molecular Medicine

ISSN: 1528-3658