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Table 1 IL-4R expression and cytotoxicity of IL-4(38-37)-PE38KDEL on human breast carcinoma cell lines and primary cell cultures

From: Human Breast Carcinoma Cells Express Type II IL-4 Receptors and Are Sensitive to Antitumor Activity of a Chimeric IL-4-Pseudomonas Exotoxin Fusion Protein in vitro and in vivo

Cells

IL-4(38-37)-PE38KDEL IC50 a (ng/ml)

IL-4R Expressiond (binding sites/cell)

Primary breast tumor cell cultures:

  

R-BT

0.2 ± 0.03b

954 ± 59

S-BT

5 ± 3

NDe

W-BT

240c

ND

K-BT

1000

ND

Breast cancer cell lines:

  

MCF-7

0.4 ± 0.13

2263 ± 157

BT-20

75 ± 7

1703 ± 144

SK-BR3

<1000

ND

ZR75-1

7 ± 5

4687 ± 118

MDA-MB231

1.8 ± 1.6

4598 ± 167

  1. For cytotoxicity assays, 1 × 104 cells were cultured with IL-4-toxins for 20 hr at 37°C, pulsed with 1 μCi of [3H]-leucine and further incubated for 4 hr. Cells were harvested and counted as described under Materials and Methods.
  2. aIC50, the concentration of IL-4-toxin at which 50% inhibition of protein synthesis is observed, compared with untreated cells.
  3. bThe values are presented as mean ± SEM of five experiments performed in quadruplicate.
  4. cSingle experiment performed in quadruplicate.
  5. dSingle saturating concentration of 125I-IL-4 was used to calculate binding sites. Results are shown as mean ± SD.
  6. eND = not done
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Molecular Medicine

ISSN: 1528-3658