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Fig. 2 | Molecular Medicine

Fig. 2

From: Toxicity Associated with Repeated Administration of First-Generation Adenovirus Vectors Does Not Occur with a Helper-Dependent Vector

Fig. 2

Altered transgene expression following repeated administration of first generation adenoviral vectors. (A) The experimental protocol used for the experiments described in Figure 2B and 2C. The mouse genotypes and the symbol associated with each treatment group are indicated to the left. Days are indicated on the timeline. Arrows indicate the days of vector administration. The vectors administered for each treatment group are listed below the arrows. All vector doses were 1 × 109 PFU/animal (2 × 1010, 8 × 109, 3 × 1010, and 8 × 1010 particles/animal for AdhAATΔE1, AdβgalΔE1, AdJM17ΔE1, and AdhAFPΔE1E3, respectively). (B) Levels of hAAT after the dose of AdhAATΔE1 that was administered on day 1. Treatment groups correspond to those of Figure 2A. Next to each treatment group symbol is listed the percentage of hAAT expression at day 7 (in parentheses) compared with the initial day 3 value. The number of animals (n) for each group at the start of the separate experiments is also listed. For each separate time point, significant differences among the groups receiving multiple injections and the mice receiving a single injection are indicated by an asterisk (*). The statistical test was a one-way analysis of variance (p = 0.05) with correction for multiple comparisons (Student-Newman-Keuls method). When the normality or equal variance tests failed, the comparison was conducted using the Kruskal-Wallis one-way analysis of variance on ranks with the Dunn procedure for pairwise multiple comparisons. (C) Levels of hAAT levels after the second administration of AdhAATΔE1 (day 82). Percentages and n-values are listed as described in Figure 2B. For both Figures 2B and 2C, error bars depict the standard deviation. Note the change in scale for both the X and Y axes between Figures 2B and 2C. Statistics are as described for Figure 2B.

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