Fig. 3
From: In Vivo Analysis of DNase I Hypersensitive Sites in the Human CFTR Gene
RT-PCR products generated using human CFTR-specific primers. (A, C, E) RT-PCR products generated from template RNA extracted from transgenic mouse duodenum (D), ileum (I), kidney (K), liver (Li), lung (Lu), pancreas (P), spleen (Sp), testis and genital ducts (T), Calu3 cells (Cal), and normal mouse ileum (NM). (B, D, F) A no-RNA control and no-reverse transcriptase controls for each tissue. Panels A and B show RT-PCR products generated using the primers A1R and 6S1L; C and D show products using the primers C1R and C2L; E and F show products using primers F1R and F2L. RT-PCR products were electrophoresed in a 1% agarose gel. Molecular weight (MW) is the 1 kb DNA ladder (Gibco BRL). (G) The 607 bp RT-PCR products generated from the same transgenic mouse RNA samples using the β-actin primers β-3′ and β-5′.