Table 1 Primers used for PCR
From: In Vivo Analysis of DNase I Hypersensitive Sites in the Human CFTR Gene
Primer | Primer Sequence (5′ to 3′) | Location a | Annealing Temperature (°C) | Extension Time (min) | Product Size (bp) |
|---|---|---|---|---|---|
MOU4-Ava | CCCCCGGGATCCTTCTGGATGATACAGAACC | −79.5 kb | 60 | 2 | 214 |
MOU8-Eco | GGAATTCGGATCCTATGACATGAAGACCAACC | ||||
HUG20C | CCCCCGGGATCCATCCTAACTCACAGGCAAC | −20.5 kb | 60 | 2 | 274 |
HUG20D | GAATTCGGATCCTTGATAGAGGACGAGATAC | ||||
TSR7-PacI | CCTTAATTAAGGATCCTCATCTTTATCTTCATTGTC | 185 + 10 kb | 60 | 2 | 199 |
TSR8-SacII | TCCCCGCGGATCCTAACTCATTGTACTGACGAG | ||||
4i-5 | TCACATATGGTATGACCCTC | Exon 4 | 60 | 3 | 438 |
4i-3 | TTGTACCAGCTCACTACCTA |