In Vivo Analysis of DNase I Hypersensitive Sites in the Human CFTR Gene

Table 3 Summary of RT-PCR and DHS results^a

	185 + 10 kb DHS	−20.5 kb DHS	RT-PCR mRNA	In Situ Hybridization (18)	Adult Human Expression^c	Adult Mouse Expression^c
Small intestine	+	+	+	+ + +	+ + + +	+ + + +
Colon	NA	+	NA	+	+ +	+ +
Stomach	NA	+	NA	−	+	+
Kidney	+	+	−	NA	+ +	+ +
Lung	−	(+)	+	−	+	+
Liver	+	+	−	NA	+	+
Pancreas	−	(+)	+	−	+ + +	+
Testis	−	−	+^b	NA	+	+ +
Genital ducts	−	−	+^b	−	+ +	+ +
Spleen	NA	−	−	NA	NA	NA

^a+, presence and −, absence of DHS or CFTR mRNA, respectively. NA, not analysed.
^bCombined testis and genital duct RNA were used for RT-PCR analysis (these data are for primer sets C1/C1L and F1R/F2L, not A1R/6S1L).
^cFrom previously published data.

ISSN: 1528-3658