Fig. 2
From: Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination In Vitro
Southern blot analyses of virus DNA and sequence of recombination joint. (A, B) Eight percent of cell lysate derived 9 days after infection of 293 cells was used to reinfect cells which were harvested after 2–3 days. DNA was extracted from infected 293 cells, digested by PmeI and analyzed by Southern blot analysis. Lanes 1 and 4: NheI-digested pAdCMVlacZloxP (25 ng); lanes 2 and 5: PmeI-digested sub360 wild-type adenovirus DNA (25 ng); lanes 3 and 6: PmeI-digested lacZ-adenovirus DNA (1 µg). The band intensities of wild-type sub360 and recombinant lacZ-adenovirus were similar as demonstrated by ethidium bromide staining. (A) lacZ probe; (B) adenoviral El probe. (C) DNA was extracted from CsCl-purified virus, and a recombination joint was sequenced with a primer (5′AAGTGATCGGGCCTCAGCTC-3′) to confirm the precise recombination. The loxP sequence is underlined.