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Fig. 3 | Molecular Medicine

Fig. 3

From: Efficient Generation of Recombinant Adenoviral Vectors by Cre-lox Recombination In Vitro

Fig. 3

Production of lacZ adenovirus monitored by β-galactosidase staining. Cre-treated DNA (5 µg) was transfected into 293 cells (with a transfection efficiency of ∼30% by β-galactosidase staining), and cells were harvested at the indicated times after transfection. Eight percent of the cell lysate from day 9 was used to infect 1 × 106 293 cells. Two days later, cell lysates were prepared (day 9–2). The titers of the viruses are presented as lacZ expression forming units (efu)/ml on 293 cells. The efu/ml is equivalent to titers determined by plaque assays.

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