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Fig. 1 | Molecular Medicine

Fig. 1

From: Regulation of Macrophage Migration Inhibitory Factor (MIF) Expression by Glucose and Insulin in Adipocytes In Vitro

Fig. 1

Glucose-dependent induction of MIF mRNA expression in 3T3-L1 adipocytes. Total RNA (20 µg each) was extracted from cells and MIF mRNA expression was analyzed by Northern blot as described in Materials and Methods. Cells were incubated (A) in medium A containing 1% FCS and 5 µg/ml of insulin for 24 hr with 0, 50, 100, 200, and 400 mg/dl of glucose, and (B) in medium A containing 1% FCS without insulin for 24 hr with 0, 25, 50, 100, 200, and 400 mg/dl of glucose.

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