Fig. 2

Insulin-dependent induction of MIF mRNA expression in 3T3-L1 adipocytes. Total RNA (20 µg each) was extracted from cells and MIF mRNA expression was analyzed by Northern blot as described in Materials and Methods. Cells were incubated (A) in medium A containing 1% FCS for 24 hr with 0, 0.1, 1, 5, and 10 µg/ml insulin, (B) in medium A in the absence or presence of 100 mg/dl glucose containing 1% FCS with 0 and 5 µg/ml insulin for 24 hr, and (C) in medium A with 1% FCS and 5 µg/ml insulin for 0, 1, 2, 4, 8, 12, and 24 hr.