Fig. 4From: Regulation of Macrophage Migration Inhibitory Factor (MIF) Expression by Glucose and Insulin in Adipocytes In VitroEffect of cytochalasin B on MIF secretion from adipocytes. 3T3-L1 adipocytes were treated with 5 µg/ml of insulin for 12 hr, and then the medium was changed to one containing 20 µM of cytochalasin B for 12 hr. (A) MIF protein levels in the media were determined with ELISA (n = 4). Data are shown as the mean ± SD. *p < 0.05. (B) Total RNA was obtained from cells and then MIF mRNA expression was analyzed by Northern blot.Back to article page