Fig. 5From: Regulation of Macrophage Migration Inhibitory Factor (MIF) Expression by Glucose and Insulin in Adipocytes In VitroEffect of pioglitazone on MIF secretion from adipocytes. 3T3-L1 adipocytes were incubated in medium A containing 1% FCS with or without 5 µM of pioglitazone for 24 hr. (A) MIF protein levels in media were determined with ELISA (n = 7). Data are shown as the mean ± SD. *p < 0.05. (B) Total RNA was obtained from cells and then MIF mRNA expression was analyzed by Northern blot.Back to article page