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Fig. 6 | Molecular Medicine

Fig. 6

From: Regulation of Macrophage Migration Inhibitory Factor (MIF) Expression by Glucose and Insulin in Adipocytes In Vitro

Fig. 6

Differential expression of MIF mRNA in adipose tissues of diabetic and nondiabetic rats. (A) Total RNA was obtained from epididymal fat pads of 10-week-old Wistar fatty (n = 3) and lean littermate rats (n = 3). (B) Epididymal fat pads from 30-week-old (n = 2) and 46-week-old (n = 3) OLETF and LETO rats were carefully separated into adipocytes and vascular-stromal fractions as described in Materials and Methods, and then total RNA (20 µg each) was obtained from adipocyte fractions. MIF mRNA expression was analyzed by Northern blot. Representative results of each group are shown.

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Molecular Medicine

ISSN: 1528-3658