Fig. 3
From: Loss of NF-κB Activity during Cerebral Ischemia and TNF Cytotoxicity
Western blot analysis of nuclear extracts confirms loss of NF-κB activity, followed by later up-regulation in surrounding tissues. Cortical nuclear extracts prepared from ischemic (I) and contralateral nonischemic (C) zones, and from ipsilateral surrounding area (S). Sham-operated and contralateral cortical tissues constitutively expressed NF-κB protein within nuclei. Focal cerebral ischemia induced a significant loss of NF-κB within the ischemic area; at 5 days after MCAO, surrounding infarct areas displayed the up-regulation of activated NF-κB.