Fig. 6
From: Loss of NF-κB Activity during Cerebral Ischemia and TNF Cytotoxicity
Inhibition of NF-κB activity increases TNF-mediated neurotoxicity. Control neuronal-like cell line, HTB 11, displayed 100% of viability (A); treatment with TNF (0.1 ng/ml for 24 hr) induced apoptotic cell death (11%) (B); MG-132 (10 µM for 24 hr) was moderately cytotoxic (41% cell death); (C) co-treatment with TNF and MC-132 induced significant apoptotic cell death (>75%); (D). TUNEL staining with hematoxylin and eosin. In contrast, glial cell cultures (HTB 14) were not sensitive to treatment with TNF alone, but co-treatment with MG-132 significantly increased apoptotic cell death (E). For HTB 11: TNF treatment was 0.1 ng/ml for 24 hr; MG-132 (10 µM) was added 30 min before TNF. For HTB 14: TNF treatment was 1 ng/ml for 24 hr; MG-132 (20 µM).