Skip to main content
Fig. 3 | Molecular Medicine

Fig. 3

From: TNF-α Induces Actin Cytoskeleton Reorganization in Glomerular Epithelial Cells Involving Tyrosine Phosphorylation of Paxillin and Focal Adhesion Kinase

Fig. 3

Western analysis of expression (A) and tyrosine phosphorylation (B) of focal adhesion kinase (FAK) and paxillin. (A) FAK and paxillin were immunoprecipitated with the corresponding anti-FAK and anti-paxillin antibody (Ipt: protein) from GEC that were serum starved overnight and then incubated with TNF-α (10 ng/ml) for the indicated time periods. After separation of immunoprecipitates on SDS-polyacrylamide gels, proteins were transferred to a nitrocellulose membrane and immunoblotted with monoclonal anti-FAK and anti-paxillin antibodies, respectively. (B) GEC were serum starved overnight and then treated with TNF-α (10 ng/ml) for the indicated time periods. Cell lysates were immunoprecipitated with anti-phosphotyrosine antibody (Ipt: P-Tyr). After separation of immunoprecipitates on SDS gels, proteins were transferred to a nitrocellulose membrane and immunoblotted with monoclonal anti-FAK and anti-paxillin antibodies, respectively.

Back to article page

Molecular Medicine

ISSN: 1528-3658