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Table 1 Effect of TNF-α on actin polymerization dynamics of GEC

From: TNF-α Induces Actin Cytoskeleton Reorganization in Glomerular Epithelial Cells Involving Tyrosine Phosphorylation of Paxillin and Focal Adhesion Kinase

  

TNF-α

 
 

Control 15 min (n = 10)

15 min (n = 7)

2 hr (n = 10)

4 hr (n = 9)

24 hr (n = 9)

Control 24 hr (n = 5)

G-actin (µg/mg)

20.67 ± 0.82

14.45 ± 1.52

17.9 ± 1.26

27.03 ± 2.84

83.71 ± 4.8

52.70 ± 5.9

Total actin (µg/mg)

41.55 ± 1.68

35.24 ± 2.03

50.23 ± 2.17

96.54 ± 1.96

209.28 ± 7.9

109.80 ± 10.1

G/total actin

0.50 ± 0.03

0.41 ± 0.01*

0.36 ± 0.015*

0.28 ± 0.05*

0.4 ± 0.025**

0.48 ± 0.03

  1. Monomeric (G) and total actin were quantified by the modified DNase I inhibition assay in extracts of untreated (control) or TNF-α-treated cells. Actin content is expressed in µg/mg total cell protein (mean values ± SEM).
  2. *p < 0.05 versus control at 15 min and **p < 0.05 versus control at 24 hr.