Fig. 4

Relation between AGE-associated fluorescence and immunoreactive AGE detected by each non-CML AGE antibody. (A) Fluorescence emission spectra of AGE-BSA (incubation times: a, 0 days; b, 8 weeks without glucose; c, 1 day with glucose; d, 3 days with glucose; e, 1 week with glucose; f, 2 weeks with glucose; g, 4 weeks with glucose; h, 8 weeks with glucose). Insert: Time course of the development of AGE-specific fluorescence (Ex = 360, nm/Em = 440 nm). (B–D) Immunoreactivity of AGE-BSA with non-CML AGE antibody obtained by 4-week (B), 8-week (C), and 12-week (D) incubation of AGE-RSA. Incubation times: 0 days (×), 1 day (□), 3 days (■), 1 week (△), 2 weeks (▲), 4 weeks (○), and 8 weeks (●).