Fig. 9From: Detection of Noncarboxymethyllysine and Carboxymethyllysine Advanced Glycation End Products (AGE) in Serum of Diabetic PatientsClearance of AGE peptide by hemodialysis. Pooled low-molecular-weight fractions (<10,000) from serum samples of 10 subjects (5 ml from each subject) obtained before (open symbols) and after (closed symbols) hemodialysis were subjected to gel filtration chromatography using a Sephadex G-15 column (1.5 × 110 cm). Eluted fractions were monitored for non-CML AGE (◇ or ◆) and CML (□ or ■) by ELISA (data from triplicate determinations), AGE fluorescence (△ or ▲), and absorbance at 280 nm (○ or ●). Elution of cytochrome c (V0), vitamin B12 (1355 Da), and cytidine (243 Da) (molecular weight makers) is indicated by arrows. Chromatography was monitored by absorbency at 280 nm (A) and by AGE fluorescence (Ex = 360 nm/Em = 440 nm) (B). Chromatography with affinity-purified non-CML AGE antibody was obtained using 8-week (C) and 12-week (D) AGE-RSA.Back to article page