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Fig. 2 | Molecular Medicine

Fig. 2

From: Differences in Proteinase K Resistance and Neuronal Deposition of Abnormal Prion Proteins Characterize Bovine Spongiform Encephalopathy (BSE) and Scrapie Strains

Fig. 2

Immunoblot depicting the difference in long-term stability of PrPSc fibrils during proteinase K digestion. Scrapie strains ME7, 22A, and Chandler were propagated in C57BL/6 mice. Fibrils were incubated with 50 µg/ml (final concentration) proteinase K for 1, 3, 6, 24, and 48 hr. Proteinase K activity of samples taken at each time point was halted by the addition of PMSF (final concentration, 5 mM) followed by heating at 95°C for 15 min. Samples were run on 16% SDS-PAGE gels and immunoblotted using the polyclonal anti-peptide antibody Ra5/7 to mouse PrP (residues 95–110).

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