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Fig. 5 | Molecular Medicine

Fig. 5

From: Differences in Proteinase K Resistance and Neuronal Deposition of Abnormal Prion Proteins Characterize Bovine Spongiform Encephalopathy (BSE) and Scrapie Strains

Fig. 5

Relative amounts of PrPSc in different scrapie and BSE strains or isolates. Proteins of pooled brain homogenates (10%) were separated by SDS-PAGE gel runs (16%) after proteinase K digestion and immunoblotted using a rabbit anti-peptide serum to mouse PrP sequences. For quantification of the signals, protein bands were scanned on a chemiluminescence photoimager. Reciprocal values from homogenates of different mouse lines infected with the appropriate scrapie and BSE strains or isolates to give identical signal intensities are depicted. Overall percentages were calculated as arithmetical means ± standard error of the means (SEM) of individual mice. The following numbers of mice and SDS-PAGE were included into each calculation: C57BL/6 mice were infected with Hessen1 (homogenates of 5 mice divided up into 31 gels), S833 (4 mice/16 gels), S540 (4 mice/26 gels), ME7 (5 mice/27 gels), 22A (5 mice/17 gels), 79A (6 mice/23 gels). Chandler (6 mice/39 gels), BSE-G (5 mice/30 gels), and BSE-UK (5 mice/24 gels). VM95 mice were inoculated with 87V (6 mice/32 gels), 22A (4 mice/16 gels), BSE-G (4 mice/22 gels) and BSE-UK (4 mice/21 gels). The Chandler strain was used to infect both CD-1 (8 mice/43 gels) and Tg20 mice (4 mice/16 gels). Syrian hamsters (SH) were inoculated with 263K (4 hamsters/16 gels).

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