Fig. 2
From: Development of a Facile Fluorescent Assay for the Detection of 80 Mutations Within the p53 Gene
Graph of example test data with confidence limits. Data were produced from wild-type DNA and samples harboring 1% mutant p53 sequences (238 tat). Each data point was acquired from a separate amplification reaction plate. The solid lines represent the lower and upper 95% confidence limits for the lines of best-fit for the wild-type and mutant data, respectively. For simplicity the data were assumed to be representative of a continuous population whose y variance is independent of x.