Fig. 3
From: Development of a Facile Fluorescent Assay for the Detection of 80 Mutations Within the p53 Gene
Use of the assay on tumor and associated margin tissue. The Cts from the tumor and margin fall within the expected 97.5% confidence limits for all of the 80 tests except that in well 20, 175 cac. The mutation is clearly present as a larger proportion of the p53 sequences isolated from the tumor, but is also present within the tumor margin, where its presence implies a high probability of tumor tissue remaining within the patient after surgery.