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Table 2 Sequences of control primers used in the p53 assay

From: Development of a Facile Fluorescent Assay for the Detection of 80 Mutations Within the p53 Gene

Wells

Exon

Primer (5′ to 3′)

Reverse PCR Primer/Molecular Beacon

1,2,13,14

6

ccc agg gtc ccc agg cct ctg att

862-98/6aL

25,26,37,38

6

ccc agg gtc ccc agg cct ctg att

694-98/6bL

49,50,61,62

7

cac agg tct ccc caa ggc gca c

644-98/7aL

73,74,85,86

8

ttc ctt act gcc tct tgc ttc tct

629-98/8aL

  1. Key to legend:
  2. Well = position on assay plate,
  3. exon = p53 exon
  4. Primer = Forward primer sequence used to perform control reaction
  5. Reverse PCR Primer/Molecular beacon = reverse PCR primer used with forward primer and molecular beacon used to detect PCR amplification (see table 3 for sequences).
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Molecular Medicine

ISSN: 1528-3658