From: Development of a Facile Fluorescent Assay for the Detection of 80 Mutations Within the p53 Gene
Wells | Exon | Primer (5′ to 3′) | Reverse PCR Primer/Molecular Beacon |
---|---|---|---|
1,2,13,14 | 6 | ccc agg gtc ccc agg cct ctg att | 862-98/6aL |
25,26,37,38 | 6 | ccc agg gtc ccc agg cct ctg att | 694-98/6bL |
49,50,61,62 | 7 | cac agg tct ccc caa ggc gca c | 644-98/7aL |
73,74,85,86 | 8 | ttc ctt act gcc tct tgc ttc tct | 629-98/8aL |