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Table 3 Sequences of reverse primers and molecular beacons

From: Development of a Facile Fluorescent Assay for the Detection of 80 Mutations Within the p53 Gene

ID

Sequence (5′ to 3′)

629-98

gaa tct gag gca taa ctg cac cct t

643-98

cac agg tct ccc caa ggc gca c

644-98

agc ggc aag cag agg ctg ggg c

861-98

tga ctt tca act ctg tct cct tc

862-98

tct gtc atc caa ata ctc cac acg caa

694-98

acc cgg agg gcc act gac aac

5aU

ggc cgc tct tcc tac agt act ccc ctg ccc ggc c

6aL

cgc ccg gat aag atg ctg agg agg ggc cag ggc g

6bL

cgc cca gtt gca aac cag acc tca ggc ggg ggc g

7aL

cgc ccc aag tgg ctc ctg acc tgg agt ctg ggc g

7bU

ccg gcg tgt tat ctc cta ggt tgg ctc tga ctg ccg g

8aL

cgc cgc acc gct tct tgt cct gct tgc ttc ggc g

  1. Key to legend:
  2. ID = primer identifier
  3. Sequence = reverse primer and molecular beacon sequences. All molecular beacons contain 5′ FAM (6-carboxyfluorescein) fluorophore and 3′ methyl red quecher modifications. Self-complementary stem sequences are highlighted in bold.